A platform supporting generation and isolation of random transposon mutants in <i>Chlamydia trachomatis</i>.

ABSTRACT: Chlamydia species represent a paradigm for understanding successful obligate intracellular parasitism. Despite limited genetic malleability, development of genetic tools has facilitated the elucidation of molecular mechanisms governing infectivity. Random mutagenesis approaches provide one of the most powerful strategies available to accomplish untargeted elucidation of gene function. Unfortunately, initial progress in transposon-mediated mutagenesis of Chlamydia has been challenging. To increase efficiency, we developed a plasmid-based system that couples conditional plasmid maintenance with a previously described strategy leveraging inducible expression of the Himar1-derived C9 transposase. Our pOri-Tn(Q) construct was maintained in Chlamydia trachomatis culti

SUBMITTER: Hawk C 

PROVIDER: S-EPMC11925237 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature