Quorum sensing LuxR proteins VjbR and BabR jointly regulate <i>Brucella abortus</i> survival during infection.

ABSTRACT: Brucella abortus maintains an N-acetyl homoserine lactone quorum sensing system that consists of two LuxR proteins, VjbR and BabR, as well as two signals, dodecanoyl (C12 AHL) and 3-oxododecanoyl (3-OXO-C12 AHL) homoserine lactone. This system regulates major virulence factors that influence the bacteria's survival during infection. We generated the first strain that lacks both LuxR proteins and found a synergistic interaction for survival in the chronic infection C57BL/6 mouse model. Transcriptomic analyses of the ∆vjbR∆babR double-deletion strain, as well as the cognate single-deletion strains, in a rich medium with vehicle control or supplemented with an AHL signal revealed large-scale genetic dysregulation in all conditions. Moreover, the double mutant maintained a

SUBMITTER: Caudill MT 

PROVIDER: S-EPMC11925318 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature