Lipopolysaccharide supramolecular organization regulates the activation of coagulation factor XII.

ABSTRACT: Lipopolysaccharides (LPS) are key bacterial membrane components that activate coagulation factor XII (FXII), establishing a critical link between bacterial infections, blood coagulation, and inflammation. This study investigates how the supramolecular organization of LPS-monomers, micelles, and bilayers-affects FXII activation. We demonstrate that LPS micelles uniquely activate FXII to its enzymatic form (FXIIa), while monomeric LPS modulates FXIIa activity without direct activation, and bilayer-form LPS does not induce FXII activation. The addition of calcium ions (Ca²⁺) promoted the formation of bilayers by binding to the negatively charged phosphate groups of LPS, reducing electrostatic repulsion and stabilizing LPS aggregates, potentially leading to a shift in their net char

SUBMITTER: Lira AL 

PROVIDER: S-EPMC11925649 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature