Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production.

ABSTRACT: Extensive usage of plant-based oils, especially palm oil, has led to environmental and social issues, such as deforestation and loss of biodiversity, thus sustainable alternatives are required. Microbial oils, especially from Yarrowia lipolytica, offer a promising solution because of their similar composition to palm oil, low carbon footprint and ability to utilise low-cost substrates. In this study, we employed the Design-Build-Test-Learn (DBTL) approach to enhance lipid production in Y. lipolytica. We systematically evaluated predictions from the genome-scale metabolic model to identify and overcome bottlenecks in lipid biosynthesis. We tested the effect of predicted medium supplements (glutamate, leucine, methionine and threonine) and genetic intervention targets, including the overexpr

SUBMITTER: Duman-Ozdamar ZE 

PROVIDER: S-EPMC11925697 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature