A Novel Sero-Specific-Gene Dependent Multiplex PCR Enhances the Discrimination of Major <i>Listeria monocytogenes</i> Serovars.

ABSTRACT: Listeria monocytogenes is a foodborne bacterial pathogen distributed worldwide. Serotyping is extensively applied in the classification of L. monocytogenes and is crucial in the early stage of epidemiological tracing. Among the 13 serovars, 1/2a, 1/2b, 1/2c, and 4b are the ones most frequently isolated. Numerous PCR-based methods have been presented, however, their target genes, lmo0737, ORF2110 and ORF2819, are prone to horizontal transfer or loss in certain strains, thus leading to incorrect serovar designation. Herein, we selected novel sero-specific genes and developed an improved multiplex PCR assay. The specificity of our assay was confirmed by the use of target and nontarget Listeria reference strains, as well as by the use of isolates yield

SUBMITTER: Wang J 

PROVIDER: S-EPMC11925748 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature