Elicitor-driven enhancement of phenolic compounds in geranium callus cultures: phytochemical profiling via LC-MS/MS and biological activities.

ABSTRACT: This research explores the effects of chitosan (CHT) and salicylic acid (SA) as elicitors on the production of phenolic and flavonoid compounds in Pelargonium graveolens Hort. Callus cultures on solid media, aiming to enhance antioxidant, anti-tyrosinase, and anti-elastase properties. Calli were treated with various concentrations of CHT (25, 50, 75, and 100 mg/mL) and SA (25, 50, 75, and 100 µM), and their phytochemical profiles were examined through LC-MS/MS analysis. The findings indicated that salicylic acid (SA) and chitosan (CHT) notably enhanced the levels of total phenolic content (TPC) and total flavonoid content (TFC). The greatest increase in TPC was seen in cultures treated with 25 µM of salicylic acid (SA₂), recording 336.80 ± 8.35 mg/100 g dry weight (DW), a

SUBMITTER: Elbouzidi A 

PROVIDER: S-EPMC11925866 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature