Isolation, characterization, and genomic analysis of a novel bacteriophage vB_Kp_XP4 targeting hypervirulent and multidrug-resistant <i>Klebsiella pneumoniae</i>.

ABSTRACT:

Introduction

Hypervirulent and multidrug-resistant Klebsiella pneumoniae (hvKP and MDR-KP) are significant public health threats. This study aimed to isolate a lytic bacteriophage targeting these high-risk strains, systematically characterize its biological properties, genomic features, and therapeutic efficacy, and establish a foundation for clinical phage therapy and novel antimicrobial development.

Methods

The phage vB_Kp_XP4 was isolated from river water using the double-layer agar plate method with the clinically isolated strain P4 as the host. Morphology was analyzed via transmission electron microscopy (TEM). Host range, pH, and thermal stability were assessed using spot assays and OD₆₃₀ measurements. One-step growth curves determined the latent peri

SUBMITTER: Peng X 

PROVIDER: S-EPMC11925924 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature