His⁷⁰ of Acetivibrio alkalicellulosi Cel5A is important for efficient hydrolysis of short cellodextrins.

ABSTRACT: Cellulose, a linear glucan linked by β-1,4 glycosidic bonds, is the most abundant renewable polysaccharide on earth. Complete enzymatic hydrolysis of cellulose liberates the readily metabolizable glucose that could be further converted to valuable biocommodities, and essential to this process are cellulases that hydrolyze the β-1,4 glycosidic bonds. Cellulases are among the most intensively studied and best understood enzymes, and many key residues have been uncovered and interrogated with respect to their functions in catalysis and/or substrate binding. However, it remains to be explored whether additional residues, especially in many poorly characterized cellulases such as processive endoglucanases, might also be functionally important. Here, we investigated a processive endoglucanase fr

SUBMITTER: Jia M 

PROVIDER: S-EPMC11926323 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature