An Enzyme-Encapsulated MOF@MOF Nanocomposite for Detecting H₂O₂ Derived From Superoxide Anion Released by Mitochondria of HeLa Cells.

ABSTRACT: In this work, a horseradish peroxidase (HRP)-encapsulated metal organic framework (MOF)@MOF nanocomposite is developed for detecting H₂O₂ converted by dismutation of superoxide anions released from live HeLa mitochondria. Initially, an HRP-polyacrylic acid cluster is incorporated on a mesoporous, peroxidase-like Cu/Co-1,4-benzenedicarboxylate (BDC) MOF platform to avoid structural change and deactivation of HRP through its interactions with MOF metal ions. Additionally, a Cu/Co-BDC(HRP)@1,3,5-benzenetricarboxyate (BTC) core-shell MOF/MOF structure, also with peroxide-like properties, serves as a protective matrix for HRP. Then, ultrathin porous carbon shells (UPCS) are adopted to improve the electrical conductivity of the MOF@MOF. The Cu/Co-BDC(HRP)@BTC|UPCS sensing p

SUBMITTER: Niu J 

PROVIDER: S-EPMC11926491 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature