Alkylated Hydroxychalcone: A Novel Matrix for Peptide Analysis by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry.

ABSTRACT: In matrix-assisted laser desorption ionization mass spectrometry, a suitable matrix is often selected for the analyte. Herein, we first developed a novel matrix, alkylated hydroxychalcone (AHC), which has properties similar to alkylated trihydroxyacetophenone (ATHAP) (Anal. Chem., 85: 9444-9448, 2013) developed as a matrix for hydrophobic peptides. However, the sample-to-sample reproducibility was low because of the poor crystallinity of AHC. The crystalline morphology of AHC changed when AHC/2,5-dihydroxybenzoic acid (DHB) was used as a binary matrix. As a result, the use of AHC/DHB improved sample-to-sample reproducibility and increased sensitivity for hydrophobic peptides. Mass imaging indicated that these results were due to an increased number of sweet spots wherein the analyte

SUBMITTER: Yamaguchi M 

PROVIDER: S-EPMC11926584 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature