Data-driven evaluation of suitable immunogens for improved antibody selection.

ABSTRACT: Antibodies are indispensable in laboratory and clinical applications due to their high specificity and affinity for protein antigens. However, selecting the right protein fragments as immunogens for antibody production remains challenging. Leveraging the Human Protein Atlas, this study systematically evaluates immunogen properties aiming to identify key factors that influence their suitability. Antibodies were classified as successful or unsuccessful based on standardized validation experiments, and the structural and functional properties of their immunogens were analyzed. Results indicated that longer immunogens often resulted in more successful but less specific antibodies. Shorter immunogens (50 residues or fewer) with disordered or unfolded regions at the N- or C-terminus and long coi

SUBMITTER: Waury K 

PROVIDER: S-EPMC11926642 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature