Characterization of Natural Organic Matter and Humic Substance Isolates by Size Exclusion Chromatography following Reduction with Sodium Borohydride.

ABSTRACT: Chemical reduction with sodium borohydride has been used for over four decades to probe the presence and function of carbonyl-containing moieties in dissolved organic matter (DOM). One of these structure-property relationships is the attenuation of UV-visible absorbance after borohydride reduction, an effect that has been observed universally across DOM of different origins. We previously demonstrated that DOM with similar bulk physicochemical properties exhibits bifurcating reactivity with borohydride depending on the source (i.e., soil vs. aquatic), as judged by the kinetics of fractional absorbance removal during reduction at a fixed borohydride:DOM mass ratio. This result and data from other studies suggest that a portion of borohydride-reducible chromophores in DOM may be inaccessible

SUBMITTER: Li H 

PROVIDER: S-EPMC11926756 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature