Conditional Control of Benzylguanine Reaction with the Self-Labeling SNAP-tag Protein.

ABSTRACT: SNAP-tag, a mutant of the O⁶-alkylguanine-DNA-alkyltransferase, self-labels by reacting with benzylguanine (BG) substrates, thereby forming a thioether bond. SNAP-tag has been genetically fused to a wide range of proteins of interest in order to covalently modify them. In the context of both diagnostic and therapeutic applications, as well as use as a biological recording device, precise control in a spatial and temporal fashion over the covalent bond-forming reaction is desired to direct inputs, readouts, or therapeutic actions to specific locations, at specific time points, in cells and organisms. Here, we introduce a comprehensive suite of six caged BG molecules: one light-triggered and five others that can be activated through various chemical and biochemical stimuli, such a

SUBMITTER: Caldwell SE 

PROVIDER: S-EPMC11926790 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature