Bacteria-Mediated Intracellular Radical Polymerizations.

ABSTRACT: Intracellular radical polymerizations allow for the direct bioorthogonal synthesis of various synthetic polymers within living cells, thereby providing a pathway to polymer-modified cells or the fermentative production of polymers. Here, we show that Escherichia coli cells can initiate the polymerization of various acrylamide, acrylic, and methacrylic monomers through an atom transfer radical reaction triggered by the activity of naturally occurring biomolecules within the bacterial cells. Intracellular radical polymerizations were confirmed by nuclear magnetic resonance spectroscopy, gel permeation chromatography of polymers extracted from the cells, and fluorescence labeling of the polymer directly inside the cells. The effect of polymerization on cell behavior and the response of

SUBMITTER: Ornati E 

PROVIDER: S-EPMC11926860 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature