Solid-State NMR Spectroscopy Investigation of Structural Changes of Mechanically Strained Mouse Tail Tendons.

ABSTRACT: Structural tissues like tendon are subjected to repeated tensile strains in vivo and excessive strains cause irreversible changes to the tissue. Large strains affect the molecular structure and organization of the extracellular matrix, and these are the parameters that drive cell behavior, including tissue repair. Here we describe a method to perform solid-state NMR spectroscopy on in situ strained tissue samples under magic-angle spinning to achieve high-resolution NMR spectra while maintaining the tissue's native hydration state. The changes observed in the NMR spectra are interpreted using quantum mechanics molecular mechanics (QM/MM) chemical shift calculations on strained collagen triple-helix structures and consideration of changes in the distribution of molecular orien

SUBMITTER: Kress T 

PROVIDER: S-EPMC11926861 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature