D-π-A Fluorophores with Strong Solvatochromism for Single-Molecule Ratiometric Thermometers.

ABSTRACT: Fluorescence thermometry has emerged as a significant area of research in the field of remote temperature sensing with high accuracy. However, the development of noninvasive and reliable small organic fluorescence thermometers (FTs) remains challenging. In this study, we developed a high-sensitivity, high-resolution small organic ratiometric FT with a solvatochromic dye and analyzed its temperature response mechanism. π-Extended fluorene-based D-π-A-type small solvatochromic dyes (FπAc, FπF, and FπVC) were synthesized and characterized. A significant solvatochromic shift of >200 nm was observed between n-hexane and tetrahydrofuran (THF) for both FπF and FπVC, with emission in THF reaching the red region (701 nm, 828 nm). This substantial solvatochr

SUBMITTER: Hori A 

PROVIDER: S-EPMC11926870 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature