Optimization for Simultaneous Determination of a Panel of Advanced Glycation End Products as Biomarkers for Metabolic Diseases.

ABSTRACT: Both dietary and endogenous reactive carbonyl species, such as methylglyoxal (MGO) and glyoxal (GO), react with proteins to generate advanced glycation end products (AGEs), which contribute to metabolic diseases. However, accurately determining individual AGEs in biological samples remains challenging due to the lack of standardized methods. In this study, we optimized and detailed procedures for AGE digestion using enzyme cocktails and separation and detection via high-resolution LC-MS/MS. For the first time, we observed that enzyme backgrounds contained higher levels of methylglyoxal-derived hydroimidazolone 1 (MG-H1) and glucosepane than mouse plasma by 1.4-3 times (e.g., 1512.55 ± 18.89 nM in enzymes vs 496.95 ± 90.91 nM in plasma for MG-H1). Using this optimized method, we quantified

SUBMITTER: Wang W 

PROVIDER: S-EPMC11926871 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature