DNA Sensing with Whispering Gallery Mode Microlasers.

ABSTRACT: Nucleic acid sensing is crucial for advancing diagnostics, therapeutic monitoring, and molecular biology research by enabling the precise identification of DNA and RNA interactions. Here, we present an innovative sensing platform based on DNA-functionalized whispering gallery mode (WGM) microlasers. By correlating spectral shifts in laser emission to changes in the refractive index, we demonstrate real-time detection of DNA hybridization and structural changes. The addition of gold nanoparticles to the DNA strands significantly enhances sensitivity, and exclusively labeling the sensing strand or a hairpin strand eliminates the need for secondary labeling of the target strand. We further show that ionic strength influences DNA compactness, and we introduce a hairpin-based system as a dual-p

SUBMITTER: Caixeiro S 

PROVIDER: S-EPMC11926956 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature