Airborne bacteria in institutional and commercial buildings in Korea: characterization with 16S rRNA gene sequencing and association with environmental conditions.

ABSTRACT: Information on microorganisms in indoor air of various institutional and commercial buildings has significant value in a public health management perspective. However, there is a lack of prior research comparing indoor airborne microbiota across different categories of those buildings. We characterized indoor airborne bacteria in 10 buildings (two for each of five categories: train station, parking garage, mart, public library, and daycare center) during summer and winter. The 16S rRNA gene in the bacterial gDNA extracted from samples was quantified using quantitative real-time polymerase chain reaction and sequenced with the Illumina MiSeq^™ platform for characterizing community composition. We collected information on temperature, relative humidity, CO₂ concentration

SUBMITTER: Kim YJ 

PROVIDER: S-EPMC11926998 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature