CaMYBA-CaMYC-CaTTG1 complex activates the transcription of anthocyanin synthesis structural genes and regulates anthocyanin accumulation in pepper (<i>Capsicum annuum</i> L.) leaves.

ABSTRACT: Anthocyanins are flavonoid-derived metabolites that contribute to plant and human health. At present, few studies have studied the biosynthesis and accumulation mechanism of anthocyanins in pepper leaves. The role of CaMYBA-CaMYC-CaTTG1 complex in anthocyanin biosynthesis in pepper leaves was studied. Yeast two-hybrid and dual-luciferase experiments showed that CaMYBA, CaMYC, and CaTTG1 could form an MYB-bHLH-WD40 (MBW) complex. They also have transcriptional activation on the anthocyanin synthesis structural genes CaCHS, CaCHI, CaF3H, CaF3'5'H, CaANS, CaDFR, and CaUFGT. Silencing CaMYBA or CaMYC could decrease the content of anthocyanin in pepper leaves. Transient overexpression of CaMYBA in tobacco indicated that CaMYB

SUBMITTER: Ma X 

PROVIDER: S-EPMC11927005 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature