Identification of a novel aminoglycoside nucleotidyltransferase gene in Morganella morganii from farm sewage.

ABSTRACT:

Background

Aminoglycosides are important broad-spectrum antimicrobial agents. When combined with β-lactam drugs, these agents can be used to treat severe infections such as those causing sepsis. Identifying additional resistance mechanisms will guarantee the successful application of aminoglycoside agents in clinical practice.

Methods

The isolate Morganella morganii A19 was obtained from a sewage sample from an animal farm by means of agar plate streaking. The agar dilution method was used to determine the minimum inhibitory concentrations (MICs) of the antimicrobial agents. Cloning of the predicted resistance gene was conducted, and its resistance function was assessed through MIC testing. The protein was expressed in E. coli, and the kinetic parameters were quantified. The

SUBMITTER: Lu J 

PROVIDER: S-EPMC11927176 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature