Serum miR-519d-3p and BMP2: potential early diagnostic markers and their mechanism in delayed fracture healing.

ABSTRACT:

Background

Delayed fracture healing (DFH) affects patients' quality of life, and there are limitations in diagnosis by CT scan. The purpose of the study is to evaluate the potential and mechanism of clinical application of miRNAs in DFH for early diagnosis and intervention.

Methods

Serum samples were obtained from delayed and normal fracture healing patients and the levels of miR-519d-3p and BMP2 were measured by RT-qPCR, and the value of both in the diagnosis of DFH was assessed by ROC curve. Cell viability and apoptosis were monitored using CCK8 kit and flow cytometry, respectively, and mRNA expression of osteogenesis and apoptosis-related genes were detected by RT-qPCR. The molecular interactions were verified using luciferase reporter gene system and RIP technique.

Re

SUBMITTER: Xiang J 

PROVIDER: S-EPMC11927254 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature