MPSeqM, a tool combining multiplex PCR and high-throughput sequencing to study the polymorphism of eight Leptosphaeria maculans avirulence genes and its application to field surveys in France.

ABSTRACT:

Context

Leptosphaeria maculans is a fungal pathogen causing stem canker of oilseed rape (Brassica napus). The disease is mainly controlled by the deployment of varieties with major resistance genes (Rlm). Rlm genes can rapidly become ineffective following the selection of virulent isolates of the fungus, i.e. with mutations, including deletions, in the corresponding avirulence genes (AvrLm). Reasoned and durable management of Rlm genes relies on the detection and monitoring of virulent isolates in field populations. Based on previous knowledge of AvrLm gene polymorphism, we developed a tool combining multiplex PCR and Illumina sequencing to characterize allelic variants for eight AvrLm genes in field L. maculans populations.

Results

We tested the method on DNA pools of 71 ch

SUBMITTER: Gautier A 

PROVIDER: S-EPMC11927322 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature