Isolation, expression, and in silico profiling of a thermostable xylanase from Geobacillus stearothermophilus strain NASA267: insights into structural features and agro-waste valorization.

ABSTRACT:

Background

Xylanase is an industrial enzyme with diverse applications, including nutritional supplements, agro-waste valorization, and paper pulp bleaching. This study aims to investigate the production of recombinant thermostable xylanase for converting plant biomass into fermentable sugars, a key step in various industrial processes.

Results

Geobacillus stearothermophilus strain NASA267, a Gram-positive, thermophilic bacterium, was identified as the top xylanase producer from samples collected in Egypt and Saudi Arabia. The xylanase gene xyl267 was successfully cloned from the NASA267 strain and heterologously expressed in E. coli under the control of a Lambda promoter. Optimal expression conditions were determined, with the highest enzyme activity (40 U/ml) achieved after

SUBMITTER: Ali SM 

PROVIDER: S-EPMC11927323 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature