Reverse transcriptional profiling: non-correspondence of transcript level variation and proximal promoter polymorphism.

ABSTRACT:

Background

Variation in gene expression between two Drosophila melanogaster strains, as revealed by transcriptional profiling, seldom corresponded to variation in proximal promoter sequence for 34 genes analyzed. Two sets of protein-coding genes were selected from pre-existing microarray data: (1) those whose expression varied significantly and reproducibly between strains, and (2) those whose transcript levels did not vary. Only genes whose regulation of expression was uncharacterized were chosen. At least one kB of the proximal promoters of 15-19 genes in each set was sequenced and compared between strains (Oregon R and Russian 2b).

Results

Of the many promoter polymorphisms, 89.6% were SNPs and 10.4% were indels, including homopolymer tracts, microsatellite repeats, and p

SUBMITTER: Brown RP 

PROVIDER: S-EPMC1192798 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature