Expressed sequence tags from the oomycete fish pathogen Saprolegnia parasitica reveal putative virulence factors.

ABSTRACT:

Background

The oomycete Saprolegnia parasitica is one of the most economically important fish pathogens. There is a dramatic recrudescence of Saprolegnia infections in aquaculture since the use of the toxic organic dye malachite green was banned in 2002. Little is known about the molecular mechanisms underlying pathogenicity in S. parasitica and other animal pathogenic oomycetes. In this study we used a genomics approach to gain a first insight into the transcriptome of S. parasitica.

Results

We generated 1510 expressed sequence tags (ESTs) from a mycelial cDNA library of S. parasitica. A total of 1279 consensus sequences corresponding to 525944 base pairs were assembled. About half of the unigenes showed similarities to known protein sequences or motifs. The S. parasitica s

SUBMITTER: Torto-Alalibo T 

PROVIDER: S-EPMC1192801 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature