Dual one-step recombinase-aided PCR for rapid detection of Candida in blood.

ABSTRACT: This study presents a novel dual one-step recombinase-aided PCR (DO-RAP) method, combined with recombinant human mannan-binding lectin protein (rhMBL; i.e., M1 protein)-conjugated magnetic bead (M1 bead) enrichment, for the early detection of Candida krusei and Candida parapsilosis bloodstream infections. Unlike previous studies that utilized the characteristic of docosane being solid at room temperature and melting above 44 °C as an impermeable barrier to separate two reaction steps, DO-RAP simplifies the process by eliminating this step. Specificity tests with the genomic DNAs from 11 bacterial strains and 3 fungi related to bloodstream infections (BSIs) confirmed no cross-reactivity, while sensitivity analysis demonstrated detection limits of 1 copy/μL for recombinant plasmids containin

SUBMITTER: Lyu X 

PROVIDER: S-EPMC11928390 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature