A large-scale sORF screen identifies putative microproteins involved in cancer cell fitness.

ABSTRACT: The human genome contains thousands of potentially coding short open reading frames (sORFs). While a growing set of microproteins translated from these sORFs have been demonstrated to mediate important cellular functions, the majority remains uncharacterized. In our study, we performed a high-throughput CRISPR-Cas9 knock-out screen targeting 11,776 sORFs to identify microproteins essential for cancer cell line growth. We show that the CENPBD2P gene encodes a translated sORF and promotes cell fitness. We selected five additional candidate sORFs encoding microproteins between 11 and 63 amino acids in length for further functional assessment. Green fluorescent protein fusion constructs of these microproteins localized to distinct subcellular compartments, and the majority showed reprod

SUBMITTER: Schlesinger D 

PROVIDER: S-EPMC11929002 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature