Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples.

ABSTRACT: Rapid and accurate detection of DNA from disease-causing pathogens is essential for controlling the spread of infections and administering timely treatments. While traditional molecular diagnostics techniques like PCR are highly sensitive, they include nucleic acid amplification and many need to be performed in centralized laboratories, limiting their utility in point-of-care settings. Recent advances in CRISPR-based diagnostics (CRISPR-Dx) have demonstrated the potential for highly specific molecular detection, but the sensitivity is often constrained by the slow trans-cleavage activity of Cas enzymes, necessitating preamplification of target nucleic acids. In this study, we present a CRISPR-Cascade assay that overcomes these limitations by integrating a positive feedback loop that enable

SUBMITTER: Lim J 

PROVIDER: S-EPMC11929484 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature