Enhanced preservation of viability and species stratification in <i>Lacticaseibacillus</i> group using levan-fortified skim milk as a cryoprotectant during freeze-drying.

ABSTRACT: Ensuring the viability and accurate stratification of Lacticaseibacillus group (LCG) species after freeze-drying is essential for their effective use as probiotics. This study investigates the use of reconstituted skim milk (RSM) as a cryoprotectant base, supplemented with fructans such as inulin and diverse forms of levan from Halomonas smyrnensis, to maintain the viability of Lacticaseibacillus casei ATCC 334, Lacticaseibacillus paracasei ATCC 25302, and Lacticaseibacillus rhamnosus ATCC 53103. Cellular viability was enhanced with levan-based cryoprotectants, motivating the use of levan-based hydrogels (gHLs) for freeze-drying LCG species. Throughout freeze-drying, the species-specific molecular masses (m/z) were preserved irrespective of the cryoprotec

SUBMITTER: Yavuz M 

PROVIDER: S-EPMC11929659 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature