Interface-edited solid-state NMR to study cell interfaces.

ABSTRACT: Cell membrane interfaces, including the glycocalyx, play a crucial role in regulating signaling and molecular interactions, yet their molecular composition remains challenging to study in intact cells. Existing techniques often require extensive sample preparation or lack specificity for probing interfacial components directly. Here, we introduce a solid-state nuclear magnetic resonance (ssNMR) tool to fingerprint the molecular structure of the cell glycocalyx in intact cells within their native environment, offering insights relevant to drug delivery, tissue engineering, and biomedical research. Building on Goldman-Shen cross-polarization (CP) experiments, which exploit proton spin diffusion to generate ¹³C spectra near cell membranes, our enhanced approach provides spectral in

SUBMITTER: Kress T 

PROVIDER: S-EPMC11929740 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature