Quantitative detection of β-glucans in Cordyceps species using a validated Congo red assay.

ABSTRACT: β-Glucan is extensively utilized in the food industry for its functional benefits, including blood glucose and lipid regulation, enhanced food texture, and a prolonged shelf life. In the pharmaceutical field, it serves as a potent immune modulator, anti-tumor agent, and vaccine adjuvant, highlighting its significant biological potential. Despite its wide-ranging applications, standardized methods for detecting β-glucan in Cordyceps species have been lacking. To address this deficiency, the current study developed a Congo Red Ultraviolet Spectrophotometry assay for β-glucan quantification. The optimal conditions for extraction were identified as dimethyl sulfoxide (DMSO) being the solvent, a pH of 7.0, a temperature of 65 °C, and a reaction time of 60 min. Validation tests confirmed the met

SUBMITTER: Xia H 

PROVIDER: S-EPMC11929836 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature