Cohesin and condensin regulate chromosome topology and play an essential role in maintaining pluripotency in embryonic stem cells.

ABSTRACT: Cohesin and condensin, two related protein complexes, play essential roles in ensuring the accurate segregation of the genome into daughter cells during cell division. However, the interaction between cohesin and condensin in embryonic stem cells remains unclear, as does the specific function of the meiosis-specific cohesin complex. Cohesin maintains the cohesion of replicated sister chromatids until their separation at anaphase, whereas condensin facilitates the reorganization of chromosomes into a highly compact structure characteristic of mitosis. First, we found via ChIP-seq analysis that cohesins (SMC3, RAD21, and REC8) and condensin (SMC4) share DNA binding sites in close proximity and directly interact with the insulator protein CTCF. Second, siRNA-regulated SMC3 depletion led to nu

SUBMITTER: Choi EH 

PROVIDER: S-EPMC11929898 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature