Swi4-dependent SWI4 transcription couples cell size to cell cycle commitment.

ABSTRACT: Growth-dependent accumulation of the G1/S transcription factor SBF, composed of Swi4 and Swi6, occurs in G1 phase in budding yeast and is limiting for commitment to division, termed Start. Here, we investigate the mechanisms for the size dependence of Swi4 accumulation using different genetic contexts and quantitative scanning number and brightness microscopy. Mutation of SBF binding sites in the SWI4 promoter or disruption of SBF activation resulted in ∼33-50% decrease in Swi4 accumulation rate and concordantly increased cell size at Start. Ectopic inducible expression of Swi4 in G1 phase cells increased production of Swi4 from the endogenous promoter, upregulated transcription of the G1/S regulon, and accelerated Start. A threshold model in which Swi4 titrates SBF binding sites in

SUBMITTER: Goswami P 

PROVIDER: S-EPMC11930368 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature