Cryo-EM structure of a phosphotransferase system glucose transporter stalled in an intermediate conformation.

ABSTRACT: The phosphotransferase system glucose-specific transporter IICB^Glc serves as a central nutrient uptake system in bacteria. It transports glucose across the plasma membrane via the IIC^Glc domain and phosphorylates the substrate within the cell to produce the glycolytic intermediate, glucose-6-phosphate, through the IIB^Glc domain. Furthermore, IIC^Glc consists of a transport (TD) and a scaffold domain, with the latter being involved in dimer formation. Transport is mediated by an elevator-type mechanism within the IIC^Glc domain, where the substrate binds to the mobile TD. This domain undergoes a large-scale rigid-body movement relative to the static scaffold domain, translocating glucose across the membrane. Structures of elevator-type tr

SUBMITTER: Roth P 

PROVIDER: S-EPMC11930441 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature