The Impact of Obesity on Autophagy in Human Adipose-Derived Mesenchymal Stromal Cells.

ABSTRACT: Mesenchymal stromal cells (MSCs) possess therapeutic properties, which can be blunted by obesity. Autophagy, a cellular recycling process, is essential for MSC function. We investigated the mechanisms by which obesity affects the properties of MSCs, with a focus on autophagy. Adipose tissue was obtained from kidney donors [body mass index (BMI) <30 kg/m², non-obese] or individuals undergoing weight loss surgery (BMI ≥30 kg/m², obese) for MSC harvesting (n = 11 each); samples were randomized to sequencing (seq; n = 5 each) or functional studies (n = 6 each). MSCs were sequenced to determine their epigenetic (5-hydroxymethylcytosine) and transcriptomic profiles across autophagy-related genes using hydroxymethylated DNA immunoprecipitation sequencing

SUBMITTER: Xing L 

PROVIDER: S-EPMC11930488 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature