Development and evaluation of protective immunity of a ROP27 DNA vaccine against Eimeria tenella in chickens.

ABSTRACT: This study aimed to develop and evaluate the protective immunity of a ROP27 DNA vaccine against Eimeria tenella (E. tenella) in chickens. E. tenella is a parasitic protozoan that poses a significant threat to the poultry industry. The rhoptry protein 27 (ROP27) of E. tenella has been shown to have immunoprotective properties. However, traditional protein expression methods are time-consuming and labor-intensive, limiting large-scale production. In this study, we developed a pVAX-ROP27 DNA vaccine and confirmed its expression in chickens using RT-PCR and Western blot analysis. The protective immunity of the DNA vaccine was evaluated through an animal experiment with different immunization doses. The results confirmed the successful construction of the pVAX-ROP27 DNA vaccine and its in vivo

SUBMITTER: Zhao W 

PROVIDER: S-EPMC11930590 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature