Generation and characterization of a tamoxifen-inducible lineage tracing tool <i>Cd2-P2A-CreERT2</i> knock-in mice.

ABSTRACT:

Introduction

The new targeted gene editing technologies, such as the CRISPR/Cas system, enable researchers to insert or delete genes at targeted loci efficiently. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temporal specificity.

Methods

Using the CRISPR/Cas9 system, we inserted the CreERT2 transgene expression cassette into the Cd2 gene locus to generate conditional Cre-driver line Cd2-CreERT2 knock-in mice, which drove the expression of CreERT2 by the endogenous Cd2 promoter. By mating the Cd2-CreERT2 strain with a Rosa26-LSL-tdTomato reporter mouse strain which contains a tdTomato expression fragment blocked with a loxP-flanked STOP cassette (LSL) driven by a CAG promoter,

SUBMITTER: Guo Y 

PROVIDER: S-EPMC11931051 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature