Combining multispectral and high-resolution 3D imaging for leaf vein segmentation and density measurement.

ABSTRACT: Accurate leaf vein segmentation and vein density (VLA) measurement are crucial for understanding plant physiology. Traditional 2D imaging techniques often require labor-intensive and destructive processes, such as leaf flattening or chemical clearing, thereby limiting their practicality for high-throughput applications. In this study, we present a novel framework that integrates multispectral and high-resolution 3D imaging to enhance leaf vein segmentation and VLA measurement. By leveraging digital fringe projection, our system captures grayscale, multispectral, and 3D topographical data within a unified coordinate system. The integration of 3D information improves vein detection, particularly in low-contrast regions, while also enabling direct and accurate measurements of leaf area, vein

SUBMITTER: Liao YH 

PROVIDER: S-EPMC11931515 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature