Inhibition of stromal MAOA leading activation of WNT5A enhance prostate cancer immunotherapy by involving the transition of cancer-associated fibroblasts.

ABSTRACT:

Background

The interaction between stromal cells and the tumor immune microenvironment (TIME) is acknowledged as a critical driver in the progression of prostate cancer (PCa). Monoamine oxidase A (MAOA), a mitochondrial enzyme that catalyzes the degradation of monoamine neurotransmitters and dietary amines, has been linked to the promotion of prostate tumorigenesis, particularly when upregulated in stromal cells. However, the detailed mechanisms of MAOA's interaction with TIME have not been fully elucidated.

Methods

We reanalyzed a single-cell sequencing dataset to evaluate the role of MAOA in the stroma, verify the impact of stromal MAOA alterations on CD8⁺ T cell responses by co-culturing stromal cells and immune cells in vitro. Furthermore, C57BL/6J mouse subcu

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC11931948 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature