Comparison and Optimization of DNA Extraction Methods for Human DNA from Dried Blood Spot Samples.

ABSTRACT:

Background/objectives

DNA extraction from dried blood spot (DBS) samples is often applied in neonatal screening programs. Although various methods to extract DNA from DBSs have been described, the optimal approach remains unclear. Therefore, this study aimed to compare and optimize extraction methods to establish a reliable and efficient protocol for human DNA extraction from DBSs.

Methods

We conducted a back-to-back comparison of five different DNA extraction methods on 20 DBS samples: three column-based kits (QIAamp DNA mini kit, High Pure PCR Template Preparation kit, DNeasy Blood & Tissue kit) and two in-house boiling methods (one using TE buffer, one using Chelex-100 resin). DNA recovery was measured with DeNovix DS-11 and ACTB qPCR. Further optimization of eluti

SUBMITTER: Van Biesen N 

PROVIDER: S-EPMC11932244 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature