Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of <i>Nocardia seriolae</i>.

ABSTRACT:

Background

Nocardiosis, an infection caused by bacterium Nocardia seriolae, contributes to severe economic losses in the aqua-cultured fish species Seriola quinqueradiata and Seriola dumerili in Japan. Phage therapy is a potential solution for combating this infection.

Materials and methods

A lytic bacteriophage infecting N. seriolae (JCM3359) was isolated from water samples collected at various fish farms. The bacteriophage was characterized by electron microscopy, host range analysis, and genomic profiling.

Results

The isolated lytic bacteriophage, NS-I (accession no. OP429095.1), had a siphovirus morphotype with a broad lytic spectrum, as it infected all the tested N. seriolae strains (15/15). Its genome was a 43,361 bp double-str

SUBMITTER: Akmal M 

PROVIDER: S-EPMC11932517 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature