In silico construction of a multi-epitope vaccine (RGME-VAC/ATS-1) against the Rickettsia genus using immunoinformatics.

ABSTRACT:

Background

Rickettsia is a genus of Gram-negative bacteria that causes various diseases, including epidemic typhus, Rocky Mountain spotted fever, and Mediterranean spotted fever. Ticks transmit these diseases and commonly found in developing regions with poor sanitation. As a result, it is difficult to estimate the number of these diseases cases, making it challenging to create prevention and diagnostic mechanisms.

Objectives

Thus, this study aimed to develop an in silico multi-epitope vaccine against Rickettsia.

Methods

Eight proteins were previously identified as potential vaccine candidates through reverse vaccinology and were screened for epitopes that bind to MHC class I and II molecules. The epitopes were then analysed for antigenicity, allergenicity, and toxici

SUBMITTER: Felice AG 

PROVIDER: S-EPMC11932644 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature