Photosystem II supercomplexes lacking light-harvesting antenna protein LHCB5 and their organization in the thylakoid membrane.

ABSTRACT: Light-harvesting protein LHCB5 is one of the three minor antenna proteins (LHCB4-6) that connect the core (C) of photosystem II (PSII) with strongly (S) and moderately (M) bound peripheral trimeric antennae (LHCIIs), forming a dimeric PSII supercomplex known as C₂S₂M₂. Plants lacking LHCB4 and LHCB6 do not form C₂S₂M₂, indicating that these minor antenna proteins are crucial for C₂S₂M₂ assembly. However, studies on antisense asLhcb5 plants suggest this may not apply to LHCB5. Using mild clear-native PAGE (CN-PAGE) and electron microscopy (EM), we separated and structurally characterized the C₂S₂M₂ supercomplex from the Arabidopsis lhcb5 mutant. When compared w

SUBMITTER: Vanska T 

PROVIDER: S-EPMC11932966 | biostudies-literature | 2025 Mar-Apr

REPOSITORIES: biostudies-literature