<i>aac(6')-Iaq</i>, a novel aminoglycoside acetyltransferase gene identified from an animal isolate <i>Brucella intermedia</i> DW0551.

ABSTRACT:

Background

Bacterial resistance to aminoglycoside antimicrobials is becoming increasingly severe due to their use as commonly prescribed antibiotics. The discovery of new molecular mechanisms of aminoglycoside resistance is critical for the effective treatment of bacterial infections.

Methods

Bacteria in goose feces were isolated by plate streaking. The identification and characterization of a novel resistance gene from the bacterial genome involved various techniques, including molecular cloning, drug susceptibility testing, protein expression and purification, and enzyme kinetic analysis. Additionally, whole-genome sequencing and phylogenetic studies were performed.

Results

Brucella intermedia DW0551, isolated from goose feces, was resistant to 35 antibiotics

SUBMITTER: Lin N 

PROVIDER: S-EPMC11932996 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature