Adaptive attenuation of virulence mediated by Wzc mutation in ST11-KL47 Carbapenem-resistant <i>Klebsiella pneumonia</i>.

ABSTRACT:

Introduction

The impact of the hypermucoviscosity (HMV) phenotype in ST11-KL47 carbapenem-resistant Klebsiella pneumoniae (CRKp) pathogenicity warrants investigation for public health risk assessment.

Methods

We analyzed 230 clinical ST11-KL47 CRKp to identify the key factor in mucoviscosity acquisition via comparative genomic analysis. Sedimentation value served as the objective index to quantify HMV. The virulence in vivo was assessed using Galleria mellonella and mouse infection models. We employed genome engineering, capsular polysaccharides (CPS) quantification, and visualization to explore the role of Wzc mutation in CPS biosynthesis and HMV. The biological impact of Wzc-mediated HMV was investigated through competitive growth analysis, biofilm for

SUBMITTER: Dai Y 

PROVIDER: S-EPMC11933079 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature