The vascular-cambium-specific transcription factor PtrSCZ1 and its homologue regulate cambium activity and affect xylem development in <i>Populus trichocarpa</i>.

ABSTRACT:

Introduction

Vascular cambium proliferates and differentiates into the secondary xylem (wood), enabling the perennial increase in stem diameter for wood formation. In our previous study, we identified 95 vascular-cambium-specific (VCS) transcription factors (TFs) in Populus trichocarpa.

Methods

In this study, we characterized the function of the highly vascular cambium-expressed heat shock TF among these VCSs, PtrSCZ1, using PtrSCZ1-overexpressing transgenic lines and gene-edited mutants in P. trichocarpa.

Results

Overexpressing PtrSCZ1 or its homolog PtrSCZ3 (OE-PtrSCZ1, OE-PtrSCZ3) led to enhanced cambium activity, increased stem diameter, and a larger xylem proportion. CRISPR-based mutants of PtrSCZ1 and Pt

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11933121 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature