Microsecond pulsed electric fields induce myocardial ablation by secondary mitochondrial damage and cell death mechanisms.

ABSTRACT: Microsecond pulsed electric field (µsPEF) is a newer treatment modality to replace catheter ablation treatment of Atrial fibrillation (AF) due to its fewer side effects. This study aims to find out experimental parameters that effectively induce cardiomyocyte death and the precise mechanisms for microsecond pulsed electric fields (µsPEFs) ablation of cardiomyocytes. CCK8 and flow apoptosis analysis were employed to examine the effects of different µsPEFs on cardiomyocytes in vitro. The mechanisms by which the µsPEFs ablation works were explored through a combination of transcriptome study, transmission electron microscope (TEM) observation of mitochondria, pathway enrichment analysis, and interaction network analysis. In vivo experiments on mice involving HE, Masson, TUNEL and Immunofluore

SUBMITTER: Gao Q 

PROVIDER: S-EPMC11933261 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature