Dynamic synthesis and transport of phenazine-1-carboxylic acid to boost extracellular electron transfer rate.

ABSTRACT: Electron shuttle plays a decisive role in extracellular electron transfer (EET) of exoelectrogens. However, neither identifying the most efficient electron shuttle molecule nor programming its optimal synthesis level that boosts EET has been established. Here, the phenazine-1-carboxylic acid (PCA) biosynthesis pathway is first constructed to synthesize PCA at an optimal level for EET in Shewanella oneidensis MR-1. To facilitate PCA transport, the porin OprF is expressed to improve cell membrane permeability, the cytotoxicity of which, however, impaired cell growth. To mitigate cytotoxicity, PCA biosensor is designed to dynamically decouple PCA biosynthesis and transport, resulting in the maximum output power density reaching 2.85 ± 0.10 W m^-2, 33.75-fold higher than wild-type st

SUBMITTER: Li F 

PROVIDER: S-EPMC11933291 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature