Non-viral generation of transgenic non-human primates via the piggyBac transposon system.

ABSTRACT: Non-human primates, such as cynomolgus monkeys, are invaluable experimental models for understanding human biology and disease. Their close genetic relationship to humans makes them essential for studying fundamental human developmental processes and disease progression. Although lentiviral methods for generating transgenic monkeys exist, several inherent technical difficulties limit their utility. To solve this problem, here we establish a non-viral method for generating transgenic cynomolgus monkeys using the piggyBac transposon system. After optimizing our protocol in mice, we show that the co-injection of piggyBac components with sperm into metaphase II-stage oocytes successfully generates transgenic monkeys expressing transgenes throughout their whole bodies. Transgene expression is o

SUBMITTER: Nakaya M 

PROVIDER: S-EPMC11933304 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature